Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1
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منابع مشابه
Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1.
Fluorescent protein (FP)-based biosensors have revolutionized the ability of researchers to monitor enzyme activities in live cells. While the basic design principles for FP-based biosensors are well established, first-generation biosensor constructs typically suffer from relatively low fluorescence responses that limit their general applicability. The protein engineering efforts required to su...
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p13(suc1) (Cks) proteins have been implicated in the regulation of cyclin-dependent kinase (CDK) activity. However, the mechanism by which Cks influences the function of cyclin-CDK complexes has remained elusive. We show here that Cks1 is required for the protein kinase activity of budding yeast G(1) cyclin-CDK complexes. Cln2 and Cdc28 subunits coexpressed in baculovirus-infected insect cells ...
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ژورنال
عنوان ژورنال: Mol. BioSyst.
سال: 2014
ISSN: 1742-206X,1742-2051
DOI: 10.1039/c3mb70402e